Figure 3.
Effect of Meth/sex pretreatment on conditioned cue-induced pERK. (a) Experimental design and timeline of experiment 2, showing 4 days of Saline/sex or Meth/sex treatment, followed 2 weeks later by 8 conditioning trials of conditioned sex aversion (CSA), and the final exposure to home cage or female for phosphorylated MAP kinase (pERK) analysis. (b,c) Numbers of pERK-immunoreactive neurons per mm2 (mean±SEM) in males pretreated with Saline/sex (saline), or Meth/sex (Meth) and removed from home cage (control; white bars; n=12 saline and n=10 Meth) or exposed to the female (female; black bars; n=14 saline and n=17 Meth). (b) pERK in mPFC subregions and OFC, and (c) in NAc, CPU and CeA. ACA, anterior cingulate area; PL, prelimbic cortex; IL, infralimbic cortex; OFC, orbitofrontal cortex; NAc, nucleus accumbens; CPU, caudate putamen; VTA, ventral tegmental area; LA, lateral amygdala; BLA, basolateral amygdala; CeA, central amygdala. *Significant difference from home cage control group, #Significant difference from Saline/sex-pretreated home cage control group.