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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Pain. 2017 Sep;158(9):1711–1722. doi: 10.1097/j.pain.0000000000000966

Figure 6.

Figure 6

A: Intraplantar administration of URB597 decreased evoked responses in C-fiber nociceptors isolated from HbSS-BERK sickle mice. Data show the mean (±SEM) number of impulses evoked by 147 mN before and at 30, 60, 90 and 120 min after various drug treatments. The number of evoked impulses was reduced at 30, 60, 90, and 120 min following intraplantar administration of URB597, and this effect was blocked by the CB1 receptor antagonist AM281. Pre-treatment with the CB2 receptor antagonist AM630 did not alter the effect of URB597, and these fibers exhibited lower responses to 147 mN at 30, 60, 90, and 120 min after injection. *p<.05, **p<.005, ***p≤.001 vs. the vehicle-treated group. ###p<.001 indicates significant differences from pre-injection value.

B: Representative examples of responses of individual C-fibers evoked by 147 mN for 5 s before (pre-injection) and at 30, 60, 90 and 120 min after intraplantar injection of either vehicle, URB597, URB597 + AM281, or URB597 + AM630. The time of mechanical stimulation is illustrated at the bottom of each column. Evoked activity was reduced by URB597 and this was blocked by the CB1 receptor antagonist AM281.