Table 1.
Antibodies
| Antibody name | Immunogen | Manufacturer and catalog #, RRID, species, mono- or poly-clonal |
Dilution (with PBS) |
|---|---|---|---|
| Cleaved-Casp3 | Endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. |
Cell Signaling, 9661, AB_2341188, rabbit, polyclonal |
1:1600 |
| Crx | Amino acids 166 –285 mapping near the C-terminus of CRX of human origin. |
Santa Cruz Biotechnology, sc- 30150, AB_2276566, rabbit, polyclonal), |
1:100 |
| Nr2e3 | VPS36 fusion protein ag5549 | Proteintech, 14262-1-AP, rabbit, polyclonal |
1:100 |
| Opn1mw/lw | Extracellular doman of human OPN1LW; mouse OPN1MW |
Santa Cruz Biotechnology, sc- 22117, AB_2300956, goat, polyclonal |
1:100 |
| Opn1sw | N-terminus of the opsin protein encoded by OPN1SW |
Santa Cruz Biotechnology, sc- 14363, AB_2158332, goat, polyclonal |
1:100 |
| Rhodopsin 4D2 | N-terminal region of rhodopsin | Gift from Dr. Robert S. Molday from University of British Columbia, Canada, AB_2315273, mouse, monoclonal |
1:100 |
| Synaptophysin | A synaptosome prepared from rat retina. |
Sigma-Aldrich, s5768, AB_477523, mouse, monoclonal |
1:400 |
| Top2b (Topo IIbeta) |
Amino acids 1341–1626 of Topo IIβ of human origin. |
Santa Cruz Biotechnology, sc- 25330, AB_628384, mouse, monoclonal |
1:300 |
| Tuj-1 | microtubules derived from rat brain | Abcam, ab14545, mouse, monoclonal |
1:1000 |
| vGlut1 | Strep-Tag® fusion protein of rat VGLUT 1 (aa 456 – 560) |
Synaptic Systems, 135302, AB_887877, rabbit, polyclonal |
1:300 |