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. 2017 Aug 11;8:16016. doi: 10.1038/ncomms16016

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Representative pictures and averaged data of re-endothelialization. Re-endothelialization was quantified in Evans blue-stained carotid arteries at 3 and 7 days after vascular injury. Blue staining indicates endothelial denudation. n=9 per group. Scale bar, 10 mm. (b) mRNA levels of SRSF1 in human coranary artery endothelia cells (HCAECs) treated with Ang II at 6, 12 and 24 h; n=8 per group. (c) SRSF1 expression in HCAECs treated as in a; n=7 per group. (d) Overexpression of SRSF1 by Ad-SRSF1 infection in HCAECs; n=5 per group. (e) Δ133p53 levels in HCAECs infected with Ad-GFP or Ad-SRSF1; n=6 per group. (f) Overexpression of Δ133p53 in cultured HCAECs by Ad-Δ133p53 infection; n=5 per group. (g) Representative images and averaged data from wound-healing assays in HCAECs infected with Ad-GFP, Ad-SRSF1 or Ad-Δ133p53 and treated Ang II; n=9 per group. Scale bar, 200 μm. (h) Δ133p53 siRNAs (Δ133 si1 and Δ133 si2) deplete Δ133p53 protein in HCAECs; n=6 per group. (i) Representative images and averaged data from wound-healing assays in HCAECs infected with Δ133p53 siRNAs with or without Ad-SRSF1 overexpression and treated with Ang II; n=9 per group. Scale bar, 200 μm. (j,k) PCNA expression in cultured HCAECs infected with Ad-SRSF1 (j) or Ad-Δ133p53 (k); n=5 each. (l) MTT assays of HCAECs infected with Ad-GFP, Ad-SRSF1 or Ad-Δ133p53 after Ang II for 4 days; n=12 per group. (m) KLF5, p21, and PCNA levels in HCAECs infected with scrambled or Δ133p53 siRNAs in the presence or absence of Ad-SRSF1 overexpression; n=7 per group. (n) MTT assays showing the SRSF1-mediated enhancement of proliferation was inhibited by Δ133p53 knockdown in HCAECs; n=15 per group. Adenoviral infection above is 100 m.o.i. for 48 h. Ang II concentration is 200 nM. Scr indicates scrambled siRNA control. *P<0.05, **P<0.01, one-way ANOVA (a–c,e,g–n). Data are mean±s.e.m. of four independent experiments (a–c,e,g–n).