Table 1.

Predicted secondary metabolite loci in Z. tritici

Cluster number	Secondary metabolite class	Predicted key biosynthetic gene(s)	Predicted transporter	Resident transcription factor	Subtelomeric	Evidence of transcriptional co-expression?	AntiSMASH		SMURF
							Cluster boundary	No. of genes	Cluster boundary	No. of genes
1	NRPS-Like	Mycgr3G107072	YES	NO	NO	NO	1:2,172,553:2,212,738	12	1:2,189,682:2,216,244	8
2	Transporter	Na	YES	NO	NO	NO	1:2,306,586:2,310,597	2	Na	Na
3	Terpene	Mycgr3G34236	NO	NO	NO	YES	1:2,366,583:2,438,578	28	Na	Na
4	Terpene	Mycgr3G33174	YES	NO	NO	NO	1:3,518,416:3,535,837	9	Na	Na
5	PKS	Mycgr3G83965	NO	NO	NO	NO	1:3,821,874:3,860,702	14	Na	Na
6	NRPS	Mycgr3G16590	YES	YES	NO	NO	1:5,128,643:5,192,491	23	Na	Na
7	PKS	Mycgr3G67477	YES	YES	NO	YES	1:5,513,274:5,566,181	17	1:5,524,934:5,578,932	20
8	PKS	Mycgr3G9788	YES	YES	YES	YES	2:31,258:149,728	37	2:123,001:157,676	11
9	NRPS	Mycgr3G19958	NO	NO	YES	NO	2:222,377:310,014	19	2:260,654:284,183	5
10	NRPS	Mycgr3G36951	YES	NO	NO	NO	2:420,337:471,819	14	2:433,742:453,243	2
11	NRPS	Mycgr3G90558	YES	NO	NO	NO	2:1,008,626:1,058,615	13	2:1,026,842:1,052,480	6
12	Terpene	Mycgr3G99148	NO	YES	NO	YES	2:1,934,751:2,046,004	31	Na	Na
13	PKS-Like	Mycgr3G39149	YES	NO	YES	YES	Na	Na	3:3,181,477:3,213,160	13
14	NRPS	Mycgr3G39931, Mycgr3G40534	YES	NO	YES	NO	4:12,877:43,949	7	Na	Na
15	PKS	Mycgr3G100089	YES	YES	YES	YES	5:85,463:149,465	22	5:94,206:129,292	12
16	NRPS-Like	Mycgr3G93235, Mycgr3G100227	NO	YES	NO	YES	5:1,291,545:1,380,182	31	5:1,309,417:1,324,422	4
17	PKS	Mycgr3G72709	YES	YES	YES	YES	6:72,339:170,251	36	6:106,974:121,853	4
18	NRPS	Mycgr3G72768	YES	NO	YES	YES	6:283,399:352,537	24	6:310,922:346,260	11
19	NRPS	Mycgr3G44313	NO	NO	NO	YES	6:1,808,394:1,845,239	16	6:1,821,703:1,841,259	9
20	NRPS	Mycgr3G109989	YES	YES	NO	NO	6:2,243,873:2,278,779	9	Na	Na
21	PKS	Mycgr3G45348	YES	NO	YES	NO	7:2,566,479:2,608,068	12	7:2,577,096:2,606,813	8
22	NRPS	Mycgr3G110642	YES	NO	YES	NO	8:2,346,378:2,374,211	8	8:2,352,913:2,362,665	2
23	NRPS-Like	Mycgr3G75370	YES	YES	NO	YES	9:483,924:714,190	65	Na	Na
24	PKS	Mycgr3G47832	YES	YES	NO	NO	9:1,474,679:1,516,611	12	9:1,494,054:1,506,383	3
25	Terpene	Mycgr3G76129	YES	YES	NO	NO	10:341,276:419,193	29	Na	Na
26	Hybrid PKS- NRPS	Mycgr3G62978	YES	NO	NO	YES	10:425,702:485,734	20	10:439,622:460,865	6
27	PKS	Mycgr3G101493	YES	NO	YES	YES	10:1,274,562:1,480,511	54	10:1,436,111:1,437,155	7
28	NRPS-Like	Mycgr3G49555	NO	NO	NO	YES	11:393,046:429,601	12	11:409,058:423,584	5
29	PKS	Mycgr3G96592	NO	YES	NO	NO	11:566,694:611,143	14	Na	Na
30	NRPS-Like	Mycgr3G50095	YES	YES	NO	NO	11:823,532:917,882	30	11:849,800:868,675	7
31	NRPS	Mycgr3G96900	YES	NO	YES	YES	12:157,368:195,467	15	Na	Na
32	NRPS-Like	Mycgr3G77312	NO	NO	NO	NO	12:434,096:471,899	9	Na	Na
33	PKS	Mycgr3G51018	NO	NO	NO	YES	13:656,107:712,077	17	Na	Na
34	Terpene	Mycgr3G101921, Mycgr3G12838	YES	NO	YES	NO	13:1,116,277:1,139,970	8	Na	Na

AntiSMASH and SMURF analysis pipelines were used to predict secondary metabolite clusters in Z. tritici isolate IP0323, identifying a total of 34 putative loci. Ensembl gene identifiers for predicted key biosynthetic genes identified by these analyses are given. Clusters were numbered in ascending numerical order based on their respective location in the Z. tritici genome. In order to identify genes encoding ABC or MFS transporters at each locus, genes were interrogated based on GO-terms GO:0055052 (ATP-binding cassette transporter complex) and GO:0055085 (transmembrane transporter). Transcription factors were identified by screening genes for GO-terms GO:0003677 (DNA binding) and GO:0003700 (transcription factor activity). Subtelomeric clusters were defined as any loci with predicted genes residing within 300 kb of the chromosome end. Interrogation of each individual cluster suggests the key gene of cluster 13 encodes a ketoacyl synthase domain-containing protein, and consequently is likely involved in fatty acid synthesis. Cluster 2 consists of two transport proteins, and lacks a gene encoding a putative key biosynthetic enzyme