Fig. 2.

miR-140-5p directly targets YES1 in GC cells. a miR-140-5p binding sites of YES1 3′ UTR predicted by the database Targetscan. b Expression levels of miR-140-5p determined by quantitative real-time PCR in five GC cell lines and one normal human gastric epithelial cell line GES1. c Quantification real-time PCR verification of overexpression after miR-140-5p transfection in AGS and BGC823 cells. d Western blot of YES1 protein in GC cells after miR-140-5p or control mimics transfection. e Relative luciferase activity in AGS cells co-transfected with miR-140-5p or control mimics together with reporter vectors carrying wild-type (WT) or mutated-type (MT) YES1 3′ UTR