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A time-course expression analysis of eGFP expression under control of TpSIT1 promoter in Thalassiosira pseudonana. Exponentially growing cells were transferred to silicon-deprived media (0 h) and expression of eGFP was followed by measuring eGFP fluorescence using an imaging flow cytometer (a 488-nm laser with 100 mW power output without a blocking filter). Error bars represent standard errors of mean (SEM), n = 20,000
