Fig. 6.

Effect of N-terminus presence (TpSIT1P + N term) or absence (TpSIT1p only) coding sequence of SIT1 on expression of eGFP. eGFP was expressed under the transcriptional control of TpSIT1 promoter. A 96-nt long 5’ coding sequence was cloned between promoter and eGFP (TpSIT1p + N term). eGFP was measured with a 488-nm laser with 50 mw power output without a blocking filter. Net eGFP fluorescence (b) = eGFP fluorescence/cell (a) x No. of cells expressing eGFP, depicted as % in (c). Error bars represent standard errors of mean (SEM), n = 20,000