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. Author manuscript; available in PMC: 2017 Aug 18.
Published in final edited form as: Cell Rep. 2017 Aug 8;20(6):1448–1462. doi: 10.1016/j.celrep.2017.07.036

Figure 1. Distinct neural rosette transcriptional profiles compared to 2D neural stem cells.

Figure 1

(A) Top: schematic of the neural rosette differentiation protocol. Bottom: Immunostaining for neural markers in NRs at day 11 of differentiation. (B) UCSC Genome Browser snapshot of PAX6 locus (chr11:31,800,000–31,850,000) showing normalized signals of DNA methylation level (mCG/CG), RNA-seq (read count expressed as TPM), and ChIP-seq reads (RPKM, input normalized). ChIP-seq peak calls are shown as bars above each track. (C) Heatmap representation of expression levels (TPM) of entropy-based cell-type specific protein-coding (left) and lncRNA (right) genes. See related Supplemental Experimental Procedures, Figure S1 and Table S1.