Figure 2. Effect of miR-181a antagomir and E2 on injury from middle cerebral artery occlusion (MCAO).
(A) Serum E2 levels in female mice prior to and following ovariectomy (OVX), with and without 17β-estradiol (E2) replacement. (B) Coronal brain sections stained with Cresyl Violet to assess infarct volume in OVX mice treated with mismatch control (MM) or miR-181a antagomir (Antag), and E2 or vehicle (V), 24 hr following 1 hr MCAO; infarcted areas are lighter in color. Quantification of infarct volume (C) and neurological deficit (D) 24 hr following MCAO. All graphs show mean±SE, * = p<0.05 versus pre or control injury; # = p<0.05 versus Antag or E2 alone. N = 8–9 OVX animals per group. E2 = 17β-estradiol.