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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2017 May 15;82:118–125. doi: 10.1016/j.mcn.2017.05.004

Figure 3. Effect of miR-181a inhibitor and E2 on in vitro glucose deprivation (GD) astrocyte injury.

Figure 3

(A) Representative micrographs of cell death assessed by propidium iodide (PI, red) staining following GD injury in C6 cells treated with control scramble sequence (Con) or miR-181a inhibitor, and E2 or vehicle (V). All cell nuclei are stained blue with Hoechst. Quantification of cell death by LDH release from C6 cells (B), male primary astrocyte cultures (C), and female primary astrocyte cultures (D) treated with miR-181a inhibitor, E2, or both. Blocking ERα using small interfering RNA (siRNA) abolished the protection seen with miR-181a inhibitor and E2 in both male and female cultures (C, D). N = 8 cultures per experiment. All graphs = mean±SE, * =p<0.05 versus control; # =p<0.05 versus E2 or inhibitor alone; Ψ= p<0.05 versus same condition without siRNA. E2 = 17β-estradiol Inh = inhibitor. Bar =15 μm.