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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2017 May 15;82:118–125. doi: 10.1016/j.mcn.2017.05.004

Figure 4. In vivo and in vitro ERα protein expression.

Figure 4

(A) Quantification of miR-181a levels from brains of OVX mice. Quantification of ERα protein from brains of OVX mice (B), male (C) and female (D) primary astrocyte cultures. ERα levels were significantly (p<0.05) lower in cultures co-treated with ERα siRNA (C, D). N = 8 cultures per experiment. All graphs = mean±SE, * = p<0.05 versus control; # = p<0.05 versus all other conditions. Antag = miR-181a antagomir, Con = transfection control (scrambled sequence), E2 = 17β-estradiol, Inh = miR-181a inhibitor, V = vehicle.