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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biomaterials. 2017 Jul 14;142:124–135. doi: 10.1016/j.biomaterials.2017.07.022

Figure 5. Construction of hS/PC spheroids.

Figure 5

(A-B). Spheroid diameter was adjusted by changing the initial cell seeding density (A), and the average spheroid diameter for a given cell seeding density was calculated (B, *:significantly different from spheroids with 30 µm diameter p<0.05, δ: significantly different from spheroids with 60 µm diameter p<0.05). (C) Microscopic observation of hS/PC spheroids. The bright field image shows the gross appearance of assembled structures. Confocal images were acquired on the entire spheroid as a Z-stack (ii) and on a cryosectioned slice to show the central lumen (iii). F-actin and nuclei were stained green and blue, respectively. The combined fluorescence and bright field image (iv) showed the presence of proliferative cells, stained green for Ki67.