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rEhCP112a provokes bodipy diffusion in Caco-2 cells. Caco-2 cells were apically labeled by bodipy FC sphingomyelin (green) and incubated with rEhCP112a or EDTA or refolding buffer. Cells were fixed and incubated with α-occludin antibody (red) as a TJs marker, then, cells were observed through confocal microscope in the zy-plane. Nuclei (blue) were counterstained with DAPI. Arrowheads: lipid diffused to the basolateral membranes. Arrows: occludin at TJs. Bar: 10 μm.
