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rEhCP112a alters claudin-1 and claudin-2 localization. (A–D) Confocal microscopy of Caco-2 cell monolayers incubated with rEhCP112a for 0, 2, or 30 min and then, incubated with α-EhCP112 (green) and (A) α-claudin-1 (red) or (B) α-claudin-2 (red), or (C) α-occludin (red) or (D) α-ZO-1 (red) antibodies, followed by the corresponding secondary antibodies. Nuclei were counterstained with DAPI. Arrows: co-localization. Bar: 10 μm.
