Figure 7.

rEhCP112a degrades claudin-1 and increases claudin-2 expression in Caco-2 cells. (A) Caco-2 cells incubated with rEhCP112a for different times or with live trophozoites (T) for 10 min, submitted to WB using antibodies against TJ proteins indicated at left and α-tubulin as a loading control. (B) Densitometry analysis of bands obtained in (A) for each TJs protein with regards to tubulin. ^*p ≤ 0.05, ^**p ≤ 0.001 compared to the basal condition (0 time). (C) Caco-2 cells incubated with rEhCP112a or E-64-pre-treated rEhCP112a, submitted to WB using α-claudin-1 antibody. (D) Caco-2 cells lysates from confluent and sparse cultures and submitted to WB using antibodies indicated at left. Blots are representative of at least three independent experiments.