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. 2017 Jun 14;16(2):1826–1836. doi: 10.3892/mmr.2017.6773

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Copyright: © Park et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Downregulation of EGFR and CD44 with siRNA inhibited the migration of ovarian cancer cells. (A) SK-OV-3 cells were treated with si-EGFR or si-Ctrl and EGF for 16 h. Migratory capabilities were analyzed using a wound healing assay. (B) Protein expression levels of Ras, Raf, p-MEK and total MEK were assessed using western blot analysis in SK-OV-3 cells. (C) Caov-3 cells were treated with si-CD44 or si-Ctrl and EGF or EGF combined with HA for 16 h. Migratory capabilities were analyzed using a wound healing assay. (D) Protein expression levels of Ras, Raf, p-MEK and total MEK were assessed using western blot analysis in Caov-3 cells. β-actin served as the loading control. Photomicrographs were captured at ×100 magnification using a digital camera under an inverted phase-contrast microscope. Scale bars, 100 µm. Results are representative of three independent experiments. EGF, epidermal growth factor; EGFR, EGF receptor; CD, cluster of differentiation; si, small interfering; Ctrl, control; p-, phosphorylated; MEK, mitogen-activated protein kinase/extracellular signal-regulated kinase kinase; HA, hyaluronan.