Figure 3. Salinomycin (SAL) reversed doxorubicin-induced epithelial-mesenchymal transition in cholangiocarcinoma cells. A, Western blot detection of E-cadherin and vimentin expression in control, doxorubicin- (DOX), doxorubicin plus SAL-, or SAL alone-treated cholangiocarcinoma cells. GAPDH was used as an internal control. B, Expression of CD133 detected by flow cytometry in RBE cells treated with DOX in the presence or absence of SAL. C, CCK-8 assay of the viability of RBE and Huh-28 cells following DOX and/or SAL treatment after twist siRNA interference.
