Figure 4. Effects of PMM-034 on human enterovirus 71 (EV71/VP1) mRNA and protein expressions in rhabdomyosarcoma (RD) cells. RD cells were infected with EV71 for 1 h and then treated with 10 μg/mL PMM-034 or DMSO for 23 h. A, The EV71/VP1 mRNA level was detected by real-time RT-PCR. GAPDH was used as the internal control. Data are reported as means±SD of at least 3 independent experiments, **P<0.01, Student’s t-test. B, Expression levels of EV71/VP1 protein were detected by western blotting. β-actin was used as the internal control.
