Figure 4. . Effect of TAX on EC4 DNA.

(A) Quantification of Hoechst intensity per cell and plotting the corresponding histogram reveals aneuploidy present in TAX-treated nuclei. Untreated cells reveal that a significant portion of the cells in replicating phase (labeled 4N), and rest in diploid state (2N). However, post-treatment with TAX, most cells exhibit aneuploidy (i.e., number of chromosomes greater than 4N). This effect is further enhanced by encapsulating TAX in filomicelles. Empty nanocarriers do not produce an appreciable change in this distribution. (B) Quantification of aneuploidy by flow cytometry shows similar results to IF (Figure 4A). (C) Recovery of diploidy by EC4 cells postinduction of aneuploidy after TAX treatment. Nanocarrier-loaded TAX induce more aneuploidy after treatment (day 3), and still has more DNA content after 3 days of recovery.