Fig. 6.

SOD2 inhibition by diethyldithiocarbamate significantly reduces the TMZ resistance of GBM cells. (A) A colony formation assay was performed on P#5 GBM cells treated with TMZ (T) and/or SODi (Si) at the indicated concentrations. (B) One day after treatment with TMZ and/or SODi, the activation of Rad50 in U87MG cells was measured by Western blotting. (C) U87MG TMZ-resistant cells were treated with 50 µM TMZ alone or co-treated with SODi at different doses as indicated for two and three days. Cell proliferation was then assessed using a colorimetric MTT assay. (D) The wild-type (Wt, dotted line) and resistant (TMZ-R, solid lines) A172 cells were exposed to various concentrations of TMZ and/or SODi as indicated for two days, and cell viability was measured by the trypan blue exclusion method. (*p < 0.05; **p < 0.01; ***p < 0.001).