Table II.
The basic properties of CLDN6 analyzed by using ProtParam online software.
| A, Amino acid composition of CLDN6. | |||
|---|---|---|---|
| Amino acid | Abbreviations | Number | Composition (%) |
| Alanine | Ala(A) | 24 | 10.90 |
| Arginine | Arg(R) | 6 | 2.70 |
| Asparagine | Asn(N) | 4 | 1.80 |
| Aspartate | Asp(D) | 4 | 1.80 |
| Cystine | Cys(C) | 10 | 4.50 |
| Glutamine | Gln(Q) | 9 | 4.10 |
| Glutamate | Glu(E) | 6 | 2.70 |
| Glycine | Gly(G) | 21 | 9.50 |
| Histidine | His(H) | 2 | 0.90 |
| Isoleucine | Ile(I) | 9 | 4.10 |
| Leucine | Leu(L) | 32 | 14.50 |
| Lysine | Lys(K) | 7 | 3.20 |
| Methionine | Met(M) | 6 | 2.70 |
| Phenylalanine | Phe(F) | 4 | 1.80 |
| Proline | Pro(P) | 9 | 4.10 |
| Serine | Ser(S) | 18 | 8.20 |
| Threonine | Thr(T) | 11 | 5.00 |
| Tryptophan | Trp(W) | 6 | 2.70 |
| Tyrosine | Tyr(Y) | 8 | 3.60 |
| Valine | Val(V) | 24 | 10.90 |
| B, Molecular formula, molecular weight, isoelectric point and other basic properties of CLDN6. | |||
| Parameters | Prediction results | ||
| Formula | C1054H1682N268O291S16 | ||
| Molecular weight | 23277.5 | ||
| Theoretical pI | 8.32 | ||
| Total number of atoms | 3311 | ||
| Number of amino acids | 220 | ||
| Total number of negatively charged residues | 10 | ||
| Total number of positively charged residues | 13 | ||
| Estimated half-life (mammalian reticulocytes, in vitro) | 30 h | ||
| Instability index | 42.03 | ||
| Hydrophilic | 105–118, 153–161, 205–216 | ||
| Polarity | 106–116, 142–145, 154–161 | ||
| Turn-back coefficient | 14–22, 26–34, 43–55, 60–71, 80–89, 92–97, 100–115, 133–151, 154–156, 179–186, 195–200, 213–216 | ||
| The predicted results of the overlapping area | 106–115, 154–156 | ||