Figure 3.

Representative pictures of immunohistochemical study in aortic segments at 2 weeks post-rapamycin treatment. (A) The slides were stained with TNF-α, IL-6, IL-1β and CD68. The anti-rabbit horseradish peroxidase/diaminobenzidine detection system was used to visualize the expression (brown staining). Scale bar, 50 µm. (Sham group, n=6. TAA group, n=6. RAPA group, n=8. DMSO group, n=8). (B) The quantitation of the protein content of TNF-α, IL-6, IL-1β and CD68. The immunohistologically stained slides were assessed by computerized planimetry in the aortic adventitia and aortic media. Data are presented as the mean ± standard error of mean. *P<0.05 as indicated. RAPA, rapamycin; TNF, tumor necrosis factor; IL, interleukin; IOD, integral optical density.