Table I.
Primary antibodies | ||||
---|---|---|---|---|
Antibody name | Cat. no. | Company | Species | Dilution |
MMP-2 | 87809 | Cell signal | Rabbit | 1:100 |
MMP-9 | 13667 | Cell signal | Rabbit | 1:100 |
SM α-actin | A2547 | Sigma | Mouse | 1:200 |
Calponin | sc70487 | Santa cruz | Mouse | 1:100 |
OPN | ab8448 | Abcam | Rabbit | 1:200 |
CD68 | ab125212 | Abcam | Rabbit | 1:200 |
S100A4 | ab41532 | Abcam | Rabbit | 1:400 |
TNF-α | 8184 | Cell signal | Rabbit | 1:200 |
IL-6 | 12912 | Cell signal | Rabbit | 1:400 |
IL-1β | 12703 | Cell signal | Rabbit | 1:200 |
Cell Signal, Cell Signaling Technology, Inc.; Sigma, Sigma-Aldrich; Merck KGaA; Santa Cruz, Santa Cruz Biotechnology, Inc.; Abcam; MMP, matrix metallopeptidase; SM, smooth muscle; OPN, osteopontin; TNF, tumor necrosis factor; IL, interleukin.