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. 2017 Jun 14;38(2):899–907. doi: 10.3892/or.2017.5722

Figure 2.

Figure 2.

PD-L1 regulates DNMT1 through STAT3 signaling. (A) Western blotting (left) and qPCR (right) for DNMT1 and PD-L1 in HepG2SR transfected with PD-L1 empty vector or shRNA. (B and C) Western blotting for the protein expression of p-STAT3 and total STAT3 in HepG2SR and HepG2C (B) or HepG2SR transfected with PDL1 empty vector or shRNA (C). (D) HepG2SR cells were transfected with STAT3 siRNA or its control vector for 48 h and subjected to ChIP. The change of STAT3 binding on DNMT1 promoter was assessed by qPCR. (E) 293T cells were transfected with pGL3-DNMT1 alone or plus STAT3 vectors for 48 h, followed by the measurement of luciferase activity. Data are mean ± SD, *P<0.05, **P<0.01. The data represent three independent experiments.