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. 2017 Jun 6;16(2):1248–1254. doi: 10.3892/mmr.2017.6702

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Expression of miR-203 in rat HSCs. Rat HSC-T6 cells were transfected with a miR-203 mimic, a miR-203 inhibitor or scramble miRNA. Transfection efficiency was assessed following transient transfection with (A and B) FAM-labeled NC miRNAs and (C and D) unlabeled NC miRNAs. Photomicrographs were captured under (A and C) bright field and (B and D) fluorescence microscopy. Scale bar, 100 µm. (E) Reverse transcription-quantitative polymerase chain reaction was used to assess miR-203 expression levels in HSC-T6 cells transfected with a miR-203 mimic, a miR-203 inhibitor or scramble miRNA. Data are expressed as the mean ± standard deviation of at least three independent experiments. **P<0.01. FAM, fluorescein amidite; HSC, hepatic stellate cell; miR, microRNA; NC, negative control.