FIGURE 2.

Tenacibaculum maritimum sphingomyelinase activity. Fluorescence measurement (arbitrary units) following incubation of 1.3 ng (blue line) of recombinant protein with Amplex Red Sphingomyelinase assay kit. The control conditions were as follows: negative control, 1.3 ng of boiled recombinant protein (green line); positive control, the purified sphingomyelinase from Bacillus cereus provided by the manufacturer (red line). Results correspond to the mean of triplicates and SDs are included.