FIGURE 3.

Degradation of chondroitin sulfates by T. maritimum. (A) Colonies of T. maritimum NCIMB 2154^T, T. discolor LL04 11.1.1^T, T. jejuense KCTC 22618^T, and T. soleae LL04 12.1.7^T on marine agar 2216 supplemented with 0.2% of chondroitin sulfates A (left) and C (right). (B) Activity of the recombinant protein TmCslA_PL8 on chondroitin A (blue line) and chondroitin C (red line). The release of unsaturated oligosaccharides was monitored by spectrophotometry at 232 nm using biological triplicates. The two control conditions (green lines) correspond to the same reaction mixture with a boiled TmCslA_PL8. For the clarity of the graphic, the error bars are only indicated every 10 s.