Figure 2.

(A) Targetscan software predicted that miR-7-5p binds to the 3′UTR of TFF3. (B) Luciferase reporter assay of miR-7-5p in HEK293 cells. Normal and mutated 3′UTRs from TFF3 were subcloned into the pMIR vector. miR-7-5p was cotransfected with pMIR-TFF3-wild-type 3′UTR or pMIR-TFF3-Mut 3′UTR plasmids into HEK293 cells. The percentage of relative luciferase activity was recorded. *P<0.05 vs. pMIR and pMIR-TFF3 mut. miR, microRNA; mut, mutant; TFF3, trefoil factor 3; 3′UTR, 3′untranslated region.