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. 2017 Jun 8;16(2):1200–1206. doi: 10.3892/mmr.2017.6730

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Copyright: © Guo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-7-5p negatively regulated TFF3 protein expression. (A) Quantitative-polymerase chain reaction was performed to determine the expression of miR-7-5p post-transfection of LS174T cells with miR-7-5p mimic or inhibitor. (B) Western blotting was performed to determine the expression of TFF3 protein post-transfection of LS174T cells with miR-7-5p mimic or inhibitor. Results were normalized to GAPDH. *P<0.05 vs. NC cells. NC, cells without transfection; miR-7-5p mimic NC, cells transfected with miR-7-5p mimic NC miRNA; miR-7-5p mimic, cells transfected with miR-7-5p mimics; miR-7-5p inhibitor NC, cells transfected with miR-7-5p inhibitor NC miRNA; miR-7-5p inhibitor, cells transfected with miR-7-5p inhibitor. miR, microRNA; NC, negative control; TFF3, Trefoil factor 3.