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. 2017 Jun 30;38(2):735–744. doi: 10.3892/or.2017.5769

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — CENPU knockdown affects the expression levels of downstream genes. (A) Quantitative PCR confirmation of mRNA expression of CCND3, CXCL8, FN1, IL-1A, IL-1B, IL-6, MAD2L1, PTGS2, RAC1 and TNFRSF11B in T24 cells transfected with either a control or shCENPU lentivirus. Data represent mean ± SD of relative mRNA quantity normalized to GAPDH mRNA expression. (B) Western blot analysis of protein expression of IL-1B, CXCL8, RAC1, TNFRSF11B and IL-1A. (C) Densitometric analysis of target proteins in T24 cells transfected with control or shCENPU lentivirus. **P<0.01. CENPU, centromere protein U.