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. 2017 Jun 21;16(2):2002–2008. doi: 10.3892/mmr.2017.6826

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Copyright: © Bian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — ERK signal pathway is an important in I/R-inducing injury. (A) NAD⁺ level in the left ventricle were measured. (B) TNF-α and (C) IL-6 in the serum of rats were determined by ELISA. (D) Western blot analysis of protein level of p-ERK in different groups. (E) Relative quantitative analysis of p-ERK expression. **P<0.01 and ***P<0.001 vs. Sham; ^#P<0.05 and ^##P<0.01 vs. DMSO + I/R; ^$P<0.05 vs. Y-27632 + I/R. Similar observations were obtained in three independent experiments. ERK, extracellular signal-related kinase; I/R, ischemia/reperfusion; NAD, nicotinamide adenine dinucleotide; TNF-α, tumor necrosis factor α; IL-6, interleukin 6; p-, phosphorylated; DMSO, dimethyl sulfoxide; 3-AB, 3-aminobenzamide.