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. 2017 Jun 22;16(2):1991–2001. doi: 10.3892/mmr.2017.6846

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Copyright: © Yao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Alternatively spliced genes during the osteogenic differentiation of CESCs under normoxic and hypoxic conditions were validated by semi-quantitative PCR. CESCs were induced to differentiate in osteogenic induction medium under normoxic and hypoxic conditions for 3 weeks. A total of 7 of the 10 ASGs were successfully validated by semi-quantitative PCR. β-actin was used as an internal control. CESCs, cartilage endplate-derived stem cells; PCR, polymerase chain reaction; N, normoxia; H, hypoxia; IFNGR1; interferon γ receptor 1; PAX2, paired box 2; BCAP29, B-cell receptor associated protein 29; POSTN, periostin; FXR1, FMR1 autosomal homolog 1; CACNB4, calcium voltage-gated channel auxiliary subunit β 4; TUBD1, tubulin δ 1; Incl, inclusion; Excl, exclusion.