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. 2017 Jun 9;16(2):1298–1306. doi: 10.3892/mmr.2017.6743

Figure 3.

Figure 3.

Compounds 1 and 2 inhibited lipid accumulation, and reduced TG synthesis and lipid peroxidation induced by treatment with FFAs in HepG2 cells. Human HepG2 hepatocellular carcinoma cells were treated with 1 mmol/l FFAs alone or together with compounds 1 and 2 (25 µmol/l) for 24 h. (A) Compounds 1 and 2 inhibited lipid accumulation in HepG2 cells, as demonstrated following staining with Oil Red O. Photomicrographs were captured under ×400 magnification. (a) Control cells; (b) FFA-treated cells; (c) FFA- and compound 1-treated cells; (d) FFA- and compound 2-treated cells. (B) Treatment with compounds 1 and 2 abolished the FFA-induced increase in cell lipid content. (C) Treatment with compounds 1 and 2 significantly reduced TG levels. (D) Lipid peroxidation, assessed using cellular MDA content, was significantly inhibited following treatment with compounds 1 and 2. Data are presented as the mean ± standard deviation. ###P<0.001 vs. control cells; ***P<0.001 vs. FFA-treated cells. Compound 1, flavanomarein; Compound 2, eriodictyol 7-O-β-D glucopyranoside; TG, triglyceride; FFA, free fatty acid; MDA, malondialdehyde.