Figure 4.

Compound 2 inhibited FFA-induced ROS production in HepG2 cells. Human HepG2 hepatocellular carcinoma cells were treated with 1 mmol/l FFAs alone or together with compound 2 (25 µmol/l) for 24 h. (A) DCF green fluorescence was visualized in (a) Control, (b) FFA-treated and (c) FFA- and compound 2-treated cells. Photomicrographs were captured under ×400 magnification. (B) DCF fluorescence intensity was quantified in control, FFA-treated and FFA- and compound 2-treated cells. Data are presented as the mean ± standard deviation. ^##P<0.01 vs. control cells; *P<0.05 vs. FFA-treated cells. Compound 2, eriodictyol 7-O-β-D glucopyranoside; FFA, free fatty acid; ROS, reactive oxygen species; DCF, 2′-7′-dichlorofluorescein.