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. 2017 Jun 14;16(2):1691–1700. doi: 10.3892/mmr.2017.6772

Figure 3.

Figure 3.

RAGE knockdown reduces seizure-induced p-p65 and P-gp overexpression. Specimens were collected 72 h following status epilepticus termination. (A) RAGE, p-p65 and P-gp were significantly reduced in the siRNA group compared with the EP and scrambled groups, as determined by western blotting. Densitometry analysis was performed and data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01. (B) mRNA expression levels of RAGE and MDR1A/B in the siRNA group were significantly reduced compared with the EP and Scrambled group. **P<0.01 and ***P<0.0001 vs. sham; ##P<0.01 and ###P<0.0001 vs. EP; $$P<0.01 and $$$P<0.0001 vs. Scrambled. (C) Localization of RAGE and P-gp in hippocampal region CA3 of rat brains. Blue=nuclei, green=RAGE and red=P-gp. Images were captured using ×400 magnification. Compared with the EP and scramble groups, the expression levels of RAGE and P-gp in the siRNA group were significantly reduced. RAGE was primarily localized to the nuclei of neurons and P-gp was primarily localized to blood vessels. RAGE, receptor for advanced glycation end-product; p-p65, phosphorylated-NF-κB p65; P-gp, P-glycoprotein; MDR1A and MDR1B, P-glycoprotein gene; RAGE, receptor for advanced glycation end-product; EP, pilocarpine-induced epilepsy group.