Figure 4.

Effect of MGF on gene expression of osteoclast-associated genes. (A) The mRNA expression of cathepsin K, OSCAR, RANK, ERα, ERβ, NF-κB, c-fos, and NFATc1 were analyzed by reverse transcription-polymerase chain reaction. Total RNA extracted from control OCL in the presence of macrophage colony stimulating factor (20 ng/ml) and RANK ligand (10 ng/ml) for 3 days and BMM cells treated with or without MGF (10⁻³ M) for 3 h. (B) The results are expressed as the mean ± standard deviation of three independent experiments (n=3). *P<0.05. BMM, bone marrow macrophages; MGF, mangiferin; OCL, osteoclast-like cells; OSCAR, osteoclast-associated receptor; RANK, receptor activator nuclear factor-κB; ER, estrogen receptor; NF-κB, nuclear factor-κB; NFATc1, nuclear factor of activated T-cells, cytoplasmic 1.