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. 2017 Jun 29;38(2):703–714. doi: 10.3892/or.2017.5767

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — Galangin impedes human laryngeal carcinoma TU212 and HEP-2 cell proliferation via suppressing mTOR. (A) Western blot analysis was carried out to evaluate TSC1, p-mTOR and mTOR expression in TU212 and HEP-2 cells. TSC1 and p-mTOR protein levels in (B) TU212 and (C) HEP-2 cells were quantified after western blot assays. (D) Immunofluorescence assays were carried out to determine TSC1 positive cells in TU212 and HEP-2 cells. The quantification of TSC1 positive cells in (E) TU212 and (F) HEP-2 cells is shown. The analysis was conducted in triplicate, and the results are the mean ± SEM of three independent experiments. ***P<0.001 (compared to the control/Con).