Figure 2.

Overexpression of miR-543 inhibits the growth of glioma cells in vitro. (A) qRT-PCR was used to detect miR-543 expression in U87 and U251 cells transfected with the miR-543 mimic or miR-NC. The increased expression of U87-miR-543 and U251-miR-543 was confirmed. (B) Cell proliferation was measured by CCK-8 assay, and the overexpression of miR-543 significantly inhibited cell proliferation in both U87 and U251 cells compared with control groups (P<0.01). (C) Cell colony formation ability was measured by plate colony formation assay. Overexpression of miR-543 markedly inhibited colony formation in both U87 and U251 cells (P<0.01). *P<0.01.