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. 2017 Jun 7;16(2):1157–1166. doi: 10.3892/mmr.2017.6712

Figure 1.

Figure 1.

Workflow of the multiplex ddPCR panels. (A) The PCR mixture for each assay consisted of DNA templates, primer pairs, probes, master mix and stabilizer to a final volume of 40 µl and was (B) compartmentalized into >5 million picoliter-sized droplets for independent PCR reactions. (C) The endpoint fluorescence signal of each droplet was scanned and analyzed. (D) Different fluorophores and diverse end point fluorescence intensities distinguished the droplets containing target templates into different clusters. ddPCR, picoliter-droplet digital polymerase chain reaction; WT, wild-type; 19DEL, exon 19 deletions; L858R-1, exon 21 L858R mutation (c.2573T>G); L858R-2, exon 21 L858R mutation (c.2573T>G, c.2574G>T); T790M, exon 20 T790M mutation; FAM, 6-carboxyfluorescein; VIC, green fluorescent protein.