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Multiplex picoliter-droplet digital polymerase chain reaction panels for EGFR mutation assays. Two-dimensional histograms of the (A) 4-plex and the (B) 5-plex panels. EGFR, epidermal growth factor receptor; FAM, 6-carboxyfluorescein; VIC, green fluorescent protein; WT, wild-type; 19DEL, exon 19 deletions; L858R-1, exon 21 L858R mutation (c.2573T>G); L858R-2, exon 21 L858R mutation (c.2573T>G, c.2574G>T); T790M, exon 20 T790M mutation.
