Figure 3.

The limit of blank for the multiplex ddPCR panels is generated by observing the false-positive droplet events in assays for each mutant with wild-type templates. Fragmented plasmid containing only wild-type sequence was subjected to the 4-plex and 5-plex panel in 24 replicates. Presented here are the frequency distributions of false-positive events for (A) 19DEL, (B) T790M, (C) L858R-1 and (D) L858R-2. A Poisson model was applied to fit the frequency distribution and LOB for each, as determined by the 95% confidence interval following regression analysis, which is presented by the red cutoff line. 19DEL, exon 19 deletions; L858R, exon 21 L858R mutation; T790M, exon 20 T790M mutation; LOB, limit of blank.