Figure 6.

YAP1 is the downstream target of miR-345 in HCC cells. (A) Wild-type 3-UTR of YAP1 contained the binding sequences for miR-345. (B) Forced expression of miR-345 significantly reduced the luciferase activity of wild-type instead of the mutant 3-UTR of YAP1, miR-345 knockdown significantly increased the luciferase activity of wild-type instead of mutant 3-UTR of YAP1. (C) Overexpression of miR-345 reduced YAP1 level in MHCC-97H cells. (D) Knockdown of miR-345 increased YAP1 level in Hep3B cells; n=3. T-test was performed for statistical analysis. *P<0.05.