Figure 4.

Effects of high glucose on the Akt/mTOR and Wnt/β-catenin pathway. A total of 14 days following incubation in differing concentrations of glucose, cell lysates were prepared for western blot analysis. The protein expression levels of p-mTOR and p-Akt were increased in the 11.0 and 22.0 mM glucose groups compared with control group, however, the expression levels of p-GSK-3β and β-catenin in the two HG treatment groups appeared to remain unaffected. β-actin served as the internal control. p-, phosphorylated; Akt, protein kinase B; mTOR, mammalian target of rapamycin signaling; GSK, glycogen synthase kinase.