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. 2017 Jun 27;38(2):829–836. doi: 10.3892/or.2017.5757

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Overnight nicotinic acid (NA) treatment selectively detaches U251 cells and decreases their viability. (A-H) Phase contrast images of (A-D) U251 cells, (E and F) glia and (G and H) neurons that had been incubated in control (PBS) or the indicated concentration of NA for 12 h. (I-K) Results of MTT assays displaying the viability of (I) U251 cells, (J) glia and (K) neurons treated with the indicated concentration of NA for 12 h (**P<0.01, ***P<0.001). PBS was used as a control (0 mM of NA; same as in image below). (L and M) NA treatment detaches U251 cells and preserves cell-cell adhesions, after the MTT experiment.