Figure 1.

Effect of metformin on cell survival in H9c2 cells under hyperglycemia condition. H9c2 cells were incubated in high glucose medium containing metformin in the absence or presence of chloroquine. MTT cell survival assay was performed with MTT assay kit. LDH serves as a maker of cell death was performed with LDH assay kit. Representative images of three different samples, and each experiment was repeated at least three times. Results are expressed as mean ± SD. n=3. *P<0.05 vs. Con, #P<0.05 vs. HG, **P<0.05 vs. Met1.