Figure 6.
VSV-p14 Induces Activation of Splenic T Cells and NK Cells
4T1 tumor-bearing mice (n = 9–10 per treatment group) received one intravenous injection of PBS (control) or VSV-p14 or VSV-GFP (1 × 108 PFU) on day 12, followed by similar intratumoral inoculations on days 13, 14, and 15. Spleen cells were isolated 24 hr following the final injection. The mean ± SEM number of splenic CD4 T cells (CD4+ TCRβ+), CD8 T cells (CD8+ TCRβ+), NKT cells (CD1d-tetramer+ TCRβ+), and NK cells (CD49b+ TCRβ−) (top row) and the expression of CD69 by CD4 T cells, CD8 T cells, NKT cells, and NK cells (bottom row) were assessed by flow cytometry. *p < 0.05 compared with control.