Figure 2.

ECT2 promotes the invasion of osteosarcoma cells by upregulating EMT process. (A) ECT2 had little effect on the proliferation of osteosarcoma cells according to MTT assay. (B) Matrigel-Transwell assay revealed that ECT2 overexpression enhanced cell invasion, whereas ECT2-siRNA inhibited cell invasion. (C) Western blot results showed that ECT2 overexpression elevated the protein level of N-cadherin and downregulated the E-cadherin level. All the experiments were performed for at least three times, independently. The graphs’ lines showed the standard deviation. * indicated p<0.05 by Student t-test compared with control group.