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. 2017 Jul 20;14(9):902–910. doi: 10.7150/ijms.19965

Figure 3.

Figure 3

Knockdown YAP induced apoptosis of HL-60 cells. (A) Cells apoptosis was analyzed by FCM using double staining with FITC-labeled annexin-V and propidium iodide. Cells undergoing early apoptosis are Annexin V-FITC+/PI-, whereas cells undergoing late apoptosis are Annexin V-FITC+/PI+. The percentages of late and early apoptotic cells were summed to give the total number of apoptotic cells. (B) Morphological features of the cell apoptosis were observed by Hoechst 33342 staining (×20). (C) The expression levels of PARP, cleaved PARP, Bcl-2, and Bax were determined by western blot. (D-G) Quantitative analysis was performed by measuring the relative protein expression levels of PARP, cleaved PARP, Bcl-2, and Bax to β-Actin. Data are expressed as means ± SD. *P < 0.05.