Table 2. Residues within or in the vicinity of the PSMA active site.

^* Residues are highlighted according to their proposed roles: yellow, zinc ligands; purple, substrate- or water-binding residues; blue, residues with structural roles; green, residues that stabilize arginine patch.

^† Counterpart residues listed in single-letter code for 10 species of PSMA (Sus scrofa, Mus musculus, Rattus norvegicus, Danio rerio, Caenorhabditis briggsae, A. thaliana, Magnaporthe grisea, Aspergillus nidulans, Neurospora crassa, Gibberella zeae) and for NLD2, NLDL, Sgap, AAP, CPG₂, pepT, pepV, and TfR1. Mut, mutant; ϕ, no detectable enzymatic activity; low, enzymatic activity too low for determination of kinetic parameters; ND, not determined or too low activity for determination of V_max and K_m.

^‡ Additional mutagenesis results can be found in ref. 45.